Intermediate

Part:BBa_K223027:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-07-06)


RBS + IL-6 + HlyA + TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 637
    Illegal PstI site found at 424
    Illegal PstI site found at 499
    Illegal PstI site found at 574
    Illegal PstI site found at 595
    Illegal PstI site found at 643
    Illegal PstI site found at 746
    Illegal PstI site found at 779
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 637
    Illegal NheI site found at 556
    Illegal PstI site found at 424
    Illegal PstI site found at 499
    Illegal PstI site found at 574
    Illegal PstI site found at 595
    Illegal PstI site found at 643
    Illegal PstI site found at 746
    Illegal PstI site found at 779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 637
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 637
    Illegal PstI site found at 424
    Illegal PstI site found at 499
    Illegal PstI site found at 574
    Illegal PstI site found at 595
    Illegal PstI site found at 643
    Illegal PstI site found at 746
    Illegal PstI site found at 779
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 637
    Illegal PstI site found at 424
    Illegal PstI site found at 499
    Illegal PstI site found at 574
    Illegal PstI site found at 595
    Illegal PstI site found at 643
    Illegal PstI site found at 746
    Illegal PstI site found at 779
    Illegal AgeI site found at 123
    Illegal AgeI site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 247
    Illegal SapI site found at 661


Design Notes

RBS + IL-6 + HlyA + TT


Source

RBS + IL-6 + HlyA + TT

References